Metabolic and Hereditary Factors: Are You Stone Prone?
Fig. 8.1 Urinary cystine stone crystals (M. Daudon, CRISTAL Laboratory) The first line of treatment for this condition (or, for that matter, ANY affliction of a urinary tract stone former)…
NEPHROLOGY
The Rock of Ages: Stones Have Stood the Test of Time!
Fig. 4.1 Dornier HM1 (Courtesy of Dornier MedTech) Conclusion Treatment of urinary stones changed drastically over the centuries, from ancient medical treatment and primitive dangerous surgery to the sophisticated open…
Assessment of Renal Hemodynamics and Oxygenation by Simultaneous Magnetic Resonance Imaging (MRI) and Quantitative Invasive Physiological Measurements
Fig. 1 Schematic view of the hydraulic occluder used for induction of aortic occlusion during MRI . 1 = Indistensible extension tube, 2 = sutures, 3 = distensible silicone tube, 4 = aorta. A water-filled syringe, connected to…
Magnetic Resonance Imaging (MRI) Analysis of Ischemia/Reperfusion in Experimental Acute Renal Injury
Fig. 1 Schematic view of the hydraulic occluder used for induction of renal ischemia during MRI . 1 = indistensible extension tube, 2 = sutures, 3 = distensible silicone tube, 4 = renal vein, 5 = renal artery. A water-filled…
Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats
Fig. 1 Schematic diagram of the fully implantable telemetry system for measurement of kidney tissue PO2. After implantation surgery each rat is placed on a SmartPad that enables transcutaneous recharging…
Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI
Fig. 1 Recommended chemistry setup for filling apoferritin . (a) Double manifold importing N2 gas and expelling nitrogen through a gas bubbler (b). (c) Three neck flask fitted with a…
Intravital Multiphoton Imaging of the Kidney: Tubular Structure and Metabolism
Probe Characteristic Function 2-Photon ex range Nuclear and cell structure Hoechst 33342 Labels cell nuclei blue Nuclear change (apoptosis, mitosis) 780–820 nm Propidium iodide Labels cell nuclei red, membrane impermeable…
The Isolation and Quantitation of Fetuin-A-Containing Calciprotein Particles from Biological Fluids
Fig. 1 The appearance of CPP-containing sediments and contaminating debris following centrifugation of human serum. (a) Fibrin/red cell debris following centrifugation of serum for 30 min at 10,000 × g and 4…
Laser Capture Microdissection of Archival Kidney Tissue for qRT-PCR
Fig. 1 Methyl Carnoy’s-fixed paraffin-embedded tissue section after LCM of glomeruli (black asterisk) and proximal tubules (double black asterisk). In each case demarcation of individual glomeruli and tubules with the…
Qualitative and Quantitative Analysis of Histone Deacetylases in Kidney Tissue Sections
Fig. 1 Effects of histone acetylation and deacetylation through the actions of histone acetyl transferase (HAT) and histone deacetylase (HDAC) respectively. HDAC inhibitors (HDACi) increase acetylation by inhibiting the action…
