Renal Sympathetic Denervation in Rats
Reference Published year Animal Model Treatment Study duration Blood pressure 1 2015 Wistar rat NO synthase inhibition DNx 10 weeks ↓ 2 2014 Female SHR UNx DNx 3 weeks ↓…
An In Vitro Murine Model of Vascular Smooth Muscle Cell Mineralization
Fig. 1 Phase-contrast images of nodule formation in cultured MOVAS-1 monolayers over time 3.1.2 Seeding Cells for Experiments 1. Using a sterile pipette, remove media from flask. 2….
Biopsychronology: A Method Using Live Tissue Staining to Image Cell Function in the Kidney
Fig. 1 The three steps to “biopsychronology.” In a first step a fine needle biopsy is taken (shown is an example of a rat kidney). The second step consists of…
Isolation and Propagation of Rat Peritoneal Mesothelial Cells
Antibody IgG source Dilution Application Company α-SMA Mouse 1:200 WB/IF Sigma Cytokeratin 18 Mouse 1:100 WB/IF Sigma Vimentin Mouse 1:100 WB/IF Sigma Alexa 488 Goat 1:1000 IF Invitrogen WB, western…
Vascular Calcification in Uremia: New-Age Concepts about an Old-Age Problem
Study Model Diet Renal function Osteochondrocytic marker(s) Arterial mineralization Location Mechanism Luo et al. [82] Mgp−/− mice Standard Normal ↑Col II Medial Chondrocytic Davis et al. [218] Ldlr−/− mice High…
Rat Models of Acute and/or Chronic Peritoneal Injuries Including Peritoneal Fibrosis and Peritoneal Dialysis Complications
Fig. 1 Histopathological changes in peritonitis induced by mechanical scraping or in peritonitis induced by mechanical scraping following repeated zymosan injections. Frames a, b, e, f, i, j, m, and…
Decellularization of Rat Kidneys to Produce Extracellular Matrix Scaffolds
Fig. 1 Characterization of the DC kidney scaffolds. (a) Gross appearance of harvested DC kidney scaffolds. (b) Electron microscopy observation shows intact extracellular matrix in DC kidney scaffold. Blue arrows…
Isolation and Primary Culture of Murine Podocytes with Proven Origin
Fig. 1 Genetic map of the transgenic Pod-rtTA/LC1/R26R mice. Pod-rtTA: The podocyte promoter (derived from the human podocin gene, NPHS2) drives expression of the tetracycline-inducible reverse transactivator (rtTA2-M2) in podocytes…
Propagation and Culture of Human Renal Fibroblasts
Fig. 1 Phase contrast microscopic appearance of confluent human renal fibroblasts . Cells show characteristic “fingerprint” monolayers without forming hillocks 2 Materials 2.1 General Sterile Cell Culture Materials 1. Glass…
Enhanced Imaging: NBI, PDD, SPIES
Fig. 11.1 Endoscopic view of a superficial urothelial lesion of the left renal pelvis with a fiber optic (left) and a digital (right) ureteroscope Moreover, an adequate staging of UUT-TCC…
