Each author will have their own particular style for writing. Whilst some prefer to write the paper in order of presentation (i.e. start with Introduction and finish with Conclusions), we advocate starting with the Methods and Results. The easiest chapter to write is usually the Material and Methods (or patient population etc.), and so this is the best to start with. Use sub-headings to make it easier to structure/write and easier to read. After Methods, we then focus upon the tables, images and results. These interlink and form the main body of the work. We then write the Introduction, before the Discussion and Conclusion(s). The final component to write is the Abstract and any additional short sections (e.g. take home messages, what this adds to the field, role of funding bodies etc.). When finishing the paper, remember that it is one piece of work. Make sure that each component leads into the next, and that there are no sudden surprises. The reader wants to know what they are reading and what it is likely to show them. If you have made a major discovery, then make this clear early on (or in the title).