Urinary Proteins with Post-translational Modifications

 

Software

#PSMs(peptide spectrum match)

#Peptides

#Proteins

Whole urine

MODa

39,144

6,194

1,994
 
PEAKS 6

47,857

9,878

1,606a

PTMs

MODa

7,100

1,602

734
 
PEAKS 6

20,329

3,891

1,578

In vivo PTMs

MODa

433

169

85
 
PEAKS 6

880

254

182

Percentage of in vivo PTMs in whole urine (%)

MODa

1.106

2.728

4.263
 
PEAKS 6

1.839

2.571

11.333


aIn PEAKS 6, a protein represents a group of proteins sharing all identified peptides




Table 6.2
A summary of the names, modification sizes, and modification sites of all the in vivo PTMs, as well as the number of spectra, unique peptides, and proteins with in vivo PTMs [7]













































































































In vivo PTMs
Software
∆Mass
Position
#PSMs
#Peptides
#Proteins

Oxidation or hydroxylation
MODa
16
CDKNPRY
204
105
40

PEAKS 6
15.99
DKNPRY
224
139
71

Methylation
MODa
14
CDEHKNSQRT
106
58
27

PEAKS 6
14.02
DEILNT, C-term, N-term
157
160
99

Dehydration
MODa
−18
ST
26
19
14

PEAKS 6
−18.01
STY
102
81
67

Dihydroxy
MODa
32
CLMPT
29
1
10

PEAKS 6
31.99
KPRY
32
48
30

Phosphorylation
MODa
80
DS
20
8
2

PEAKS 6
79.97
ST
192
154
57

Acetylation
MODa
42
STM(Protein N-term)
7
5
5

PEAKS 6
42.01
CST, Protein N-term
39
41

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Nov 3, 2016 | Posted by in NEPHROLOGY | Comments Off on Urinary Proteins with Post-translational Modifications

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