Reference
Published year
Animal
Model
Treatment
Study duration
Blood pressure
1
2015
Wistar rat
NO synthase inhibition
DNx
10 weeks
↓
2
2014
Female SHR
UNx
DNx
3 weeks
↓
3
2013
SHRSP
8 % NaCl diet
DNx
2–4 weeks
↓
4
2013
129S1/SvImJ mouse
UUO
DNx
10 days
N/A
5
2013
SHR/cp
Metabolic syndrome
DNx
19 weeks
↓
6
2012
Sprague-Dawley rat
UNx + aortic regurgitation
DNx
24 weeks
→
7
2011
SHR
DM induced by STZ
DNx
45 days
→
8
2010
Dahl salt-sensitive rat
UNx + 8 % NaCl diet
DNx
6 weeks
→
9
2008
Sprague-Dawley rat
Anti-Thy-1.1 nephritis
DNx
1 week
N/A
10
2008
SPRD-Cy/+ rat
ADPKD
DNx
4 weeks
↓
11
2006
Sprague-Dawley rat
Chronic AII infusion
DNx
5 weeks
↓
12
2005
Sprague-Dawley rat
UNx + DOCA-salt
DNx
47 days
↓
13
2004
Sprague-Dawley rat
DM induced by STZ
DNx
2 weeks
→
14
2002
Sprague-Dawley rat
UNx + cigarette smoke
DNx
12 weeks
→
15
2000
Sprague-Dawley rat
UNx + cyclosporine A
DNx
3 weeks
→
16
1995
Sprague-Dawley rat
5/6 nephrectomy
DR
6 weeks
↓
17
1995
Sprague-Dawley rat
5/6 nephrectomy
DR
6 weeks
↓
18
1994
Sprague-Dawley rat
NO synthase inhibition
DNx
4 weeks
↓
2 Materials
2.1 Animals and Induction of Anesthesia
1.
2.
Anesthetic: inhalational anesthetic (e.g., sevoflurane), 10 % sodium pentobarbital in saline (v/v).
3.
Warming plate (heated pad) to keep animal warm during surgery.
2.2 Approach to Renal Sympathetic Nerve
1.
Stereoscopic microscope. We use a Wild M10 (Leica, Wetzlar, Germany), but any other stereoscopic microscopes are acceptable.
2.
Surgical instrument and additional materials: fine blunt-tipped microsurgical tweezers, scissors, two microsurgery hooks (consisting of a clip and an elastic band), cotton swabs, and gauze dressings (Fig. 1).
Fig. 1
Surgical instrument and additional materials for renal denervation. (1) Fine blunt-tipped microsurgical tweezers, (2) scissors, (3) two microsurgery hooks (consisting of a clip and elastic band), (4) cotton swabs, (5) and fine-tipped cotton swabs
2.3 Renal Sympathetic Denervation
1.
2.
Denervation solution: phenol in ethanol (10 % v/v). Phenol and absolute ethanol should be high purity grade chemicals. Melt phenol with hot water, pipette it, and add in ethanol quickly to avoid re-solidification in a pipette tip (see Note 2 ).
2.4 Verification of Renal Sympathetic Denervation (a Measurement of Kidney Norepinephrine Content)
1.
Anesthetic: inhalational anesthetic and 10 % v/v of sodium pentobarbital in saline.
2.
Surgical instrument and additional materials: tweezers, scissors, cotton swabs, and 21-gauge butterfly needles (Terumo, Tokyo, Japan).
3.
50 mL plastic syringe.
4.
Propagation and Culture of Human Renal Fibroblasts
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An In Vitro Murine Model of Vascular Smooth Muscle Cell Mineralization
The Isolation and Quantitation of Fetuin-A-Containing Calciprotein Particles from Biological Fluids
Intravital Multiphoton Imaging of the Kidney: Tubular Structure and Metabolism
Ice-cold phosphate-buffered saline (PBS; pH 7.4).
Related posts:
Propagation and Culture of Human Renal Fibroblasts
Isolation and Primary Culture of Murine Podocytes with Proven Origin
Isolation and Propagation of Rat Peritoneal Mesothelial Cells
An In Vitro Murine Model of Vascular Smooth Muscle Cell Mineralization
The Isolation and Quantitation of Fetuin-A-Containing Calciprotein Particles from Biological Fluids
Intravital Multiphoton Imaging of the Kidney: Tubular Structure and Metabolism
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