The steps for this procedure are similar to epididymal aspiration ; however, much less fluid will be obtained and it will likely be bloody. After anesthesia has been delivered, the testicle should be secured between the thumb and index fingers. A 21 gauge butterfly needle attached to a 10 mL syringe can be used to aspirate fluid from the testicle until fluid is seen in the tubing and enough is obtained for its intended purpose. The needle can be redirected to aspirate more fluid. Sample should be transferred to sperm transport media for examination by an embryologist. If good quality, adequate number of sperm is found there is no need to repeat the procedure on the same side or move to the opposite side.