Fig. 3.1
(a) This view demonstrates the correct positioning as the starting 2D field of view includes the pubic symphysis (S) anteriorly and the levator plate (LP) posteriorly. Also noted are the bladder (B), uterus (U), vagina (V), anorectum (R). (b) The structures without the ultrasound background. © Shobeiri 2013
Pelvic Floor Muscle Contraction
Many patients may not know what is meant by pelvic floor muscle contraction . It is better to phrase it in a more familiar way such as “Please do a Kegel exercise contraction,” or “Pretend you are holding your urine or gas.” This maneuver shows whether or not the patient is discoordinated. Generally, a normal patient has a strong resting tone and the levator lifts slightly; a patient who has weak pelvic floor can move the levator plate to a longer distance but cannot maintain a normal woman’s resting position (Fig. 3.2) [2].
Fig. 3.2
(a) Perineal pelvic floor ultrasound. The distance between the pubic symphysis and the levator plate (the yellow line) can be measured in resting, squeeze, and Valsalva position. In 2D images a pelvic floor muscle contraction can be quantified using displacement of the bladder neck, as well as a reduction of the midsagittal diameter (anterior-posterior, AP) of the levator hiatus at the level of minimal hiatal dimensions. © Shobeiri 2013. (b) Perineal pelvic floor ultrasound. The ultrasound wide array probes are preset for abdominal imaging. As such they show the structures upside down in a non-anatomic orientation. Here the vagina is usually seen as a collapsed structure, where vaginal walls are not clearly separated by ultrasound. Anorectum is seen as an outline of hypoechoic internal anal sphincter against the midline anal mucosa, which is usually echogenic with variable echogenicity due to fold of anal mucosa. Hyperechoic external anal sphincter surrounds the hypoechoic internal sphincter. The anorectal angle is normally easily visualized and changes with dynamic maneuvers of the pelvic floor muscles. Pubic bone (P), urethra (U), bladder (B), anorectum (A), levator ani muscle/plate (LAM). The green line is the reference line of the minimal levator hiatus
Valsalva Maneuver
When the patient is asked to perform Valsalva the instructions could state, “Bear down as if you are trying to have a bowel movement.” It is important that this is the last thing you would ask the patient to do during a dynamic ultrasonography because if she has gas in the upper rectum, it may move down and obscure the 2D/3D images you intend to obtain. If this happens, you can ask the patient to perform Valsalva again and, with the anterior movement of the levator plate, the gas and prolapse may move up cephalad. Important information can be obtained with Valsalva. Evacuation proctography used to be the gold standard to diagnose posterior compartment prolapse. A prospective study in 75 women has shown moderate to good agreement between 3D transperineal ultrasound and evacuation proctography to detect enterocele and rectocele (Fig. 3.3a). The advantage of 2D transperineal ultrasound is the view in the midsagittal plane. In this view one can visualize descent of an enterocele, the movement of the anterior rectal wall to detect a rectocele (Fig. 3.3b, c). Enterocele or, most commonly, sigmoidocele (Fig. 3.4), cystocele (Fig. 3.5), or multicompartmental defects may come to view with Valsalva maneuver. Dynamic imaging with Valsalva shows the movement of slings and meshes and may show the point of defects above, below or lateral to the mesh (Figs. 3.6 and 3.7).
Fig. 3.3
(a) Defecography demonstrating a low rectocele. (b) Perineal pelvic floor ultrasound (pPFUS) imaging with Valsalva in this patient demonstrates a low rectocele (R). The bladder (B) does not demonstrate prolapse; however, the shadow of an apical enterocele (E) is seen. Also noted are: the levator plate (LP), vagina (V), the transducer (T), and the pubic symphysis (S). © Shobeiri 2013. (c) pPFUS imaging with Valsalva in this patient demonstrates a low rectocele (R)
Fig. 3.4
Perineal pelvic floor ultrasound imaging with Valsalva in this patient demonstrates a sigmoidocele (Si). Also noted are: the levator plate (LP), anus (A), bladder (B), vagina (V), the transducer (T), and the pubic symphysis (S). © Shobeiri 2013
Fig. 3.5
Perineal pelvic floor ultrasound imaging with Valsalva in this patient demonstrates a concomitant cystocele (C) and a rectocele (R). Also noted are: vagina (V), the transducer (T), and the pubic symphysis (S). © Shobeiri 2013
Fig. 3.6
Perineal pelvic floor ultrasound imaging of a patient with anterior and posterior vaginal mesh. The anterior mesh cannot be seen as clearly. The image to the left is at rest. The double arrows point to the cephalad end of the mesh, and the single arrow points to the caudad end of the mesh. A resting rectocele (R) is seen behind the mesh. The image to the right is with Valsalva. With Valsalva the patient demonstrates worsening of the rectocele and detachment of the apical part of the mesh. Also noted are the levator plate (LP), bladder (B), the transducer (T), and the pubic symphysis (S). © Shobeiri 2013
Fig. 3.7
Perineal pelvic floor ultrasound imaging of a patient with anterior vaginal mesh. The image is with Valsalva. With Valsalva the patient demonstrates a cystocele (C) and detachment of the apical part of the mesh. The double arrows point to the cephalad end of the mesh, and the single arrow points to the caudad end of the mesh. Also noted are the levator plate (LP), bladder (B), the transducer (T), and the pubic symphysis (S). © Shobeiri 2013
3D/4D pPFUS
3D/4D ultrasound has increased public interest in the pelvic floor tremendously. The superficial axial plane faces the puborectalis portion of the levator ani, and all the levator subdivisions are better imaged by endocavitary transducers such as BK 2052 or BK 8838. However, endocavitary transducers impede Valsalva maneuver. Although the quality of perineal pelvic floor 3D/4D ultrasound images is reasonable, the end-fire endocavitary transducers used introitally have much higher resolution and may give better images.
If you have a BK ultrasound machine, the curved array transducer is capable of free hand acquisition of 3D volumes (Figs. 3.8 and 3.9). However, freehand acquisition with BK Curved Array 8802 (BK Ultrasound, Analogic, Peabody, MA, USA) is advisable only if you do not possess a BK 2052 or BK 8838. Performing freehand acquisition of a pelvic floor 3D volume takes, first, considerable skill to move the transducer at a constant speed as it sweeps radially from the patient’s right to left. Second, the acquired 3D volume is not repeatable or reliable for measurement purposes. Third, and most importantly, if you have endocavitary 16 MHz transducers at your disposal, which obtain automatic high-resolution views of the levator ani muscles (LAM) , it obviates the need for freehand perineal pelvic floor ultrasound acquisition.
Fig. 3.8
The use of the BK Curved Array 8802 transducer for freehand acquisition of 3D volumes. The transducer is placed between the labia majora and swept at a constant rate from the patient’s left to right. The time during which imaging is obtained can be set. However, slower acquisition will result in higher quality 3d volumes. © Shobeiri 2013
Fig. 3.9
(a) A 3D volume obtained using the with BK Curved Array 8802 transducer. The 3D volume can be rotated to look at different areas. (b) Demonstrated is the right sagittal view of the pelvic floor. The anterior-posterior (AP) distance defined as the shortest distance between the pubic symphysis and the levator plate is drawn in a yellow line. The AP line forms the AP line of the minimal levator hiatus (MLH) in. (c) Also noted are the levator plate (LP), bladder (B), the transducer (T), anorectum (R), anterior (A), posterior (P), caudad (C), the left-right line (LR) of the MLH, the levator ani muscle (LAM), and the pubic symphysis (PS or S). © Shobeiri 2013
The most commonly published data comes from GE Healthcare (Chicago, IL, USA) machines. Philips (Royal Philips, Amsterdam, The Netherlands), Hitachi (Hitachi Ltd., Tokyo, Japan), and others make similar or superior machines. Halo Medical Technologies (Wilmington, DE, USA) has recently introduced a low priced pelvic floor ultrasound machine. Halo does not obtain 3D images, rather video clips that can be played. GE’s 4D View is available for offline analysis and use with 4D ultrasound volumes obtained using GE’s Voluson series systems. The cheapest and most easily available system is the GE Voluson e or i (Fig. 3.10). Despite its compact size the system is very capable when used with the GE RAB4-8-RS transducer (Fig. 3.11). The systems were developed and designed to visualize surface structures of the fetus and adapted for pelvic floor imaging. GE Kretz 4D view allows manipulation of image characteristics and output of stills, cine loops, and rotational volumes in bitmap and AVI format. Slightly higher resolutions can be obtained if the GE endocavitary RIC5-9W-RS is used on the perineum (Fig. 3.12). The characteristics of these transducers are shown in Table 3.1.
Fig. 3.10
GE Voluson e ultrasound machine (GE Healthcare, Chicago, IL, USA). © Shobeiri 2013
Fig. 3.11
GE RAB4-8-RS transducer (GE Healthcare, Chicago, IL, USA). © Shobeiri 2013
Fig. 3.12
GE RIC5-9W-RS transducer (GE Healthcare, Chicago, IL, USA)
Table 3.1
Characteristics of GE RAB4-8-RS used for perineal pelvic floor ultrasound (pPFUS), and GE RIC5-9W-RS used for introital pelvic floor ultrasound (iPFUS) (GE Healthcare, Chicago, IL, USA)
Model | Description | Footprint | Bandwidth | FOV/Volume | Compatible with |
---|---|---|---|---|---|
RAB4-8-RS | Real time 4D convex transducer | 63.6 × 37.8 mm | 2–8 MHz | 70°/85° × 70° | Voluson i |
Real time 4D endocavity | |||||
RIC5-9W-RS | Next generation real time 4D micro-convex endocavitary transducer, with wide FOV | 22.4 × 22.6 mm | 4–9 MHz | 146°/146° × 120° | Voluson i |
The GE transducer is placed between the labia majora and the 2D image as outlined above is displayed on the screen. Depending on the setting of your machine, image orientation may be different. We place the ultrasound machine to the patient’s left and operate the probe with the left hand (Fig. 3.13), leaving the right hand available for running the console (Fig. 3.14). Once you have the appropriate 2D view, maximize the angle of acquisition to 75–85° and proceed with 3D imaging (Fig. 3.15). During or after acquisition of volumes it is possible to process imaging information into slices of predetermined number and spacing, reminiscent of computer tomography. This technique has been termed tomographic ultrasound imaging (TUI) by manufacturers. The combination of true 4D (volume cine loop) capability and TUI allows simultaneous observation of the effect of maneuvers. Using this methodology, the minimal levator hiatus (MLH), defined in the midsagittal plane as the shortest line between the posterior surface of the symphysis pubis and the levator plate, is the plane of reference, with 2.5 mm steps recorded from 5 mm below this plane to 12.5 mm above.
Fig. 3.13
Left-handed application of the transducer during perineal pelvic floor ultrasonography. © Shobeiri 2013
Fig. 3.14
The dominant hand generally operates the console. The GE Voluson e-buttons on the console are multifunctional, and their function corresponds to the menu at the bottom of the screen (GE Healthcare, Chicago, IL, USA). © Shobeiri 2013
Fig. 3.15
(a) 3D pelvic floor volume acquisition with a BK Curved Array 8802 transducer requires manual movement of hand to obtain a 3D volume. (b) GE RAB4-8-RS transducer has an internalized mechanism in the probe that moves the crystals, obviating the need for hand movement. The hand and the elbow should be rested in a steady position for good quality imaging. The volume obtained is displayed on the screen. Please note that the GE 3D volume in this image appears rendered (GE Healthcare, Chicago, IL, USA). © Shobeiri 2013
Fig. 3.16a reveals the obtained 3D image following the initial 2D image, using a symmetric rendered volume, of about 1.5–2.5 cm thickness, rendered caudal to cranial [10]. After capturing the 3D image, the volumes can be stored on a drive and assessed offline. The offline post-processing is done with special software that allows rotation of the volume, thick and thin slicing, and multiple measurements. During the post-processing of volumes the 3D static images are rotated to be displayed in a symmetric orientation in the three orthogonal planes: coronal, sagittal, and transverse planes. A cursor dot (colored green in Fig 3.16a) is located in corresponding positions in all three orthogonal planes. A cursor dot allows for the exact position of an anatomical structure to be identified simultaneously in the three orthogonal planes. The way to rotate the captured volume is described in four steps:
- 1.
The transverse (axial) 3D volume is rotated approximately 90° clockwise in the plane of the LAM for an appropriate anterior-posterior (AP) orientation of the image. (The plane is defined as a line joining the inferior border of the pubic symphysis and the apex of the anorectal angle as shown in Fig. 3.16b),
- 2.
The cursor dot is placed in the area of the pubic bone that allows the symphysis pubis to come into view on the coronal view,
- 3.
The coronal image is then analyzed millimeter by millimeter to identify and mark the location where the two pubic rami meet to form the inferior border of the symphysis pubis, and
- 4.
The sagittal plane is then rotated to align the inferior border of the symphysis pubis with the apex of the anorectal angle, noting that this allows the LAM to come into the full view on the transverse (axial) plane.
Fig. 3.16
3D pelvic floor volume acquisition with GE RAB4-8-RS transducer (GE Healthcare, Chicago, IL, USA). (a) Four panel view. (b) Single 3D view of the minimal levator hiatus (MLH) in the axial plane. (c) tomographic ultrasound imaging (TUI) technique, which provides the ability of multislice imaging. In the axial plane the investigator will be able to assess the entire levator ani muscle (LAM) and its attachment to the pubic rami. TUI was performed in the axial plane at 2.5 mm slice intervals, from 5 mm below the plane of minimal hiatal dimensions to 12.5 mm above, providing eight slices. (d) Left, resting, right, contracting the pelvic floor, in which the LAM appears to be much thicker, as opposed to the image obtained at rest. Moreover, we can clearly see that the anterior-posterior diameter is much smaller at pelvic floor muscle contraction, compared to at rest. This can serve as visual feedback to help women learning to perform their pelvic floor muscle exercises
The bar with the green topline in the 2D image is in the plane of the minimal hiatal dimensions after the image has been postprocessed. The visualized landmarks are: pubic bone on both sides, urethra at 12 o’clock position, vagina in the middle, anal canal at 6 o’clock position, LAM on the left and right side of the vagina, surrounding the anal canal (Fig. 3.16b–d).
GE 4D View Software
The software is available on the GE machines and also through the “Voluson Club” for Voluson ultrasound machine purchasers. Separate licenses for the software are expensive and not available to those who do not have a machine.
2D/3D/4D Introital Pelvic Floor Ultrasonography iPFUS
Basic Procedure and Equipment
Ultrasonography has become a common place in obstetrics, gynecology, and urology. Most ultrasound platforms are equipped with curved array and/or endovaginal transducers that are suitable for introital ultrasound imaging.
Positioning
As with gynecologic ultrasound, most pelvic floor ultrasound exams are performed with the woman in either lithotomy on a standard gynecologic examination table or in a modified lithotomy position with cushion placed under buttocks and lower extremities in frog-legged position. For 3D imaging the examiner may also need to prop his arm or elbow, as the imaging capture time can be as long as 15–20 s and absolute stillness is critical for the optimal image quality. It is certainly possible to do the pPFUS and iPFUS with a patient standing, which could be especially useful in patients who are not as successful with dynamic maneuver in supine position.
Probes
The latest nomenclature distinguishes two common techniques for pelvic floor ultrasound—transperineal and introital. The common denominator for all pelvic floor ultrasound techniques is placement of transducers externally on the patient’s vulva rather than introduction of the transducer into vagina or anal canal—endocavitary ultrasound.
Introital PFUS refers to ultrasound performed with the endovaginal transducer positioned at the vaginal introitus. What is now referred to as introital ultrasound can be done with use of the endovaginal transducer, which can be not only at the introitus, but also on the posterior fourchette or on the perineum, depending on the imaging goal. In this technique the endovaginal transducer used may have frequency range between 4 MHz and 9 MHz. For the purposes of this section we will focus on introital ultrasound and mention other types of ultrasound where appropriate for specific studies.
Perineal PFUS, in contrast, is performed with an abdominal transducer placed on perineal or sometimes labial majora, a technique formally referred to as perineal pelvic floor ultrasound. Perineal ultrasound uses a trans-abdominal curved array transducer, typically 4–8 MHz. It is important to keep in mind that higher frequency transducers provide superior resolution, but have less tissue penetration. This trade-off is important for achieving images of diagnostic quality.
Equipment Preparation
For pelvic floor ultrasound, as with any ultrasound imaging, use of coupling gel is a critical step, as ultrasound waves do not pass through air. Whether trans-abdominal or endovaginal transducers are used, the gel should be placed between the transducer and covering. For endovaginal transducers a disposable cover (e.g., male condom), and for curved trans-abdominal transducers a glove or plastic wrap can be used. Additionally, gel should be applied to the perineum or introitus to allow for better coupling. Warming the gel in a commercial warming device improves patient comfort. After each use, transducers should be cleaned and disinfected according to manufacturer recommendations.
iPFUS Orientation and Optimization
2D: Technique: Orientation
In introital pelvic floor ultrasound technique an end-fire endovaginal transducer is placed on the perineum with a mark facing up. The image optimization depends on the goal of desired visualization. With imaging of the pelvic floor muscles and levator hiatus the transducer is directed cranially (Fig. 3.17) and with imaging of the anorectum the transducer is usually oriented posteriorly towards the anal canal [11] (Fig. 3.18). Care should be taken to avoid excessive pressure applied to the perineal structures. Most investigators advise to assure tissue contact enough to visualize anorectal structures, avoiding any excessive pressure on the perineum. Compression of the perineum may distort perineal anatomy and limit mobility of the pelvic floor during dynamic maneuvers.
Fig. 3.17
Introital pelvic floor ultrasound. Ultrasound of the anorectal structures. (a) Schematic of endovaginal transducer positioned on the perineum and oriented caudally to visualize anorectum. (b) 2D transperineal sagittal image of the anorectum with perianal body easily visualized as an ovoid structure and anorectal angle marked
Fig. 3.18
Introital pelvic floor ultrasound. Ultrasound of the pelvic floor hiatus. (a) Schematic of endovaginal transducer positioned on the perineum and oriented cranially to visualize the pelvic floor hiatus. (b) 2D transperineal sagittal image of the pelvic floor hiatus with pubic symphysis and anorectal angle shown
In introital pelvic floor ultrasound imaging the transducer is most commonly oriented with the mark facing up (12 o’clock position) with midsagittal orientation. The produced 2D image will represent anterior structures (pubic symphysis and urethra) at the left portion of the screen and the posterior structures (anorectum) at the right side of the screen (see Fig. 3.18b). The 2D image produced by introital ultrasound imaging allows for visualization of the urethrovesical junction (UVJ) and anorectal angle, structures of the anal sphincter complex. This view can be used to observe pelvic floor mobility during pelvic floor maneuvers, like pelvic floor contraction (Kegel exercise) and during straining.
In the midsagittal view the structures seen from left to right are the pubic symphysis, urethra and bladder, vagina, and anorectum. On the midsagittal view the pubic symphysis cross-section is usually oblong, and bony structures of the pubic rami are not visible. Next, anterior and posterior urethral walls are delineated against periurethral tissues. Usually, urethral mucosa and submucosa are imaged as a universally hypoechoic structure that appears as an open lumen. The vagina is usually seen as a collapsed structure, where vaginal walls are not clearly separated by ultrasound. Anorectum is seen as an outline of the hypoechoic internal anal sphincter (IAS) against the midline anal mucosa, which is usually echogenic with variable echogenicity due to fold of anal mucosa. Hyperechoic external sphincter surrounds the hypoechoic internal sphincter. The anorectal angle is normally easily visualized and changes with dynamic maneuvers of the pelvic floor muscles. Cross-section of the puborectalis muscle (PRM) is seen posterior to the anorectal angle.