Hormone-Dependent Changes in Female Urinary Proteome



Fig. 11.1
Standard maps obtained with the analysis software PDQuest for the four comparisons investigated. The differentially expressed spots are shown as red circles a in G1 versus G2; b in G2 versus G3; c in G1 versus G3; and d in G versus G3 comparisons. From Ref. [6] with permission




Table 11.1
List of proteins identified by MS/MS analysis






















































































































































































































































































































































































































































































































































































Protein name
Gene name
Spot no.
SwissProtTrEMBL acc. #
NCBI acc. #
No. of pep identified
Mascot score
TMr. (kDa)
pI theor.
G1 versus G2 *
G2 versus G3 *
G1 versus G3 *
G versus G3 *

Alpha-1-microglobulin/bikunin
AMBP
403
P02760
gi|4,502,067
2
142
/39,886
/5.95
down 2,7
      

Aminoacylase 1
ACY1
6,401
Q03154
gi|4,501,901
9
332
/45,856
/5.77
  
down 2,13
  
down 1,78

Annexin A3, isoform CRA_b
ANXA3
5,302
P12429
gi|119,626,228
7
236
/39,845
/6.68
up 3,22
down 2,5
    

Clusterin
CLU
2,301
P10909
gi|338,305
3
151
/36,997
/5.74
up 3,53
  
up 4,31
up 2,08

Clusterin
CLU
2,302
P10909
gi|178,855
7
79
48,772
6.27
up 4,16
down 2,78
    

Clusterin
CLU
2,402
P10909
gi|178,855
5
197
/49,342
/6.27
up 2,89
  
up 2,55
  

Clusterin
CLU
3,302
P10909
gi|178,855
6
246
/48,772
/6.27
up 3,17
down 1,78
up 1,76
  

Clusterin
CLU
3,304
P10909
gi|338,305
3
184
/36,997
/5.74
up 2,43
  
up 1,69
  

Clusterin
CLU
3,401
P10909
gi|178,855
3
101
/49,342
/6.27
    
up 7,19
  

Clusterin
CLU
4,302
P10909
gi|178,855
2
107
/49,342
/6.27
up 2,67
  
up 1,97
  

Clusterin
CLU
4,304
P10909
gi|178,855
2
98
/49,342
/6.27
up 3,64
  
up 1,71
  

Beta-2 microglobulin
B2 M
7,002
P61769
gi|34,616
3
169
/12,905
/5.77
down 3,33
up 1,77
    

Complement component C4B
C4B
2,403
P0C0L5
gi|187,771
1
99
/40,795
/5.19
  
up 3,91
    

Cystatin A
CSTA
4,104
P01040
gi|4,885,165
2
96
/11,000
/5.38
  
new induced
up 9,41
up 15

Fatty acid-binding protein 5
FABP5
5,102
Q01469
gi|4,557,581
4
135
/15,155
/6.60
down 1,89
    
down 1,69

Fatty acid-binding protein 5
FABP5
5,104
Q01469
gi|4,557,581
4
135
/15,155
/6.60
down 1,61
  
down 2,78
down 2,13

Fatty acid-binding protein 5
FABP5
6,104
Q01469
gi|4,557,581
2
84
/15,497
/6.60
down 1,54
  
down 1.89
  

Gelsolin
GSN
1,403
P06396
gi|17,028,367
3
169
/31,052
/4.85
up 3
up 2,5
up 7,56
up 4,07

Gelsolin
GSN
3,403
P06396
gi|17,028,367
3
147
/31,052
/4.85
up 2,84
  
up 3,36
  

Perlecan
HSPG2
5,202
P98160
gi|11,602,963
4
208
/477,024
/6.03
  
down 2
down 2,78
down 1,89

Perlecan
HSPG2
6,103
P98160
gi|11,602,963
2
141
/477,024
/6.03
down 2,38
up 6,20
up 2,60
up 3,95

Perlecan
HSPG2
6,202
P98160
gi|184,427
3
72
/481,880
/6.05
      
down 3

Ig heavy chain variable region
IGHV3-23
7,506
  
gi|112,695,045
2
75
/10,785
/9.06
up 2,38
      

Ig heavy chain variable region
IGHV3-23
7,507
  
gi|112,695,045
2
75
/10,785
/9.06
down 1,67
      

Ig kappa light chain 1
IGKV
4,206
  
gi|4,176,418
6
397
/23,690
/6.92
down 1,89
  
down 2,22
  

Ig superfamily, member 8
IGSF8 
5,108
Q969P0
gi|16,445,029
4
206
/65,621
/8.23
down 4
  
down 2.38
  

Mannan-binding lectin-ass. serine protease 2
MASP2
4,103
O00187
gi|3,297,879
4
180
/77,176
/5.43
  
up 1,83
up 2,01
up 2,1

Prostaglandin H2 D-isomerase
PGDS
8,201
O60760
gi|32,171,249  
3
80
/21,299
/7.66
up 3,11
      

Calgranulin A   
S100A8
7,004
P05109
gi|29,888
2
89
/10,988
/9.19
down 1,70
  
down 2,86
down 2,1

Calgranulin B   
S100A9
5,001
P06702
gi|4,506,773
5
242
/13,291
/5.71
  
up 4,61
  
up 4,3

S100 calcium-binding protein A7
S100A7
6,003
P31151
gi|12,053,626
4
68
/10,410
/6.08
down 2
up 1,68
    

Serpin peptidase inhibitor, clade B, member 3
Serpin B3
7,304
P29508
gi|239,552
3
185
/44,564
/6.35
  
up 2,75
up 2,67
up 3,08

Serpin peptidase inhibitor, clade B, member 3
SerpinB3
7,305
P29508
gi|239,552
4
170
/44,564
/6.35
down 1,79
up 4,18
up 2,33
up 2,11

Serum albumin
ALB
2,706
P02768
gi|3,212,456
22
1,082
/68,425
/5.67
down 7,14
      

Serum albumin
ALB
2,707
P02768
gi|28,590
12
622
/71,246
/5.92
down 4,17
      

Serum albumin
ALB
2,708
P02768
gi|28,592
18
914
/71,316
/6.05
down 20
      

Serum albumin
ALB
3,706
P02768
gi|28,592
12
661
/71,316
/6.05
down 8,33
  
down 3,13
  

Serum albumin
ALB
3,707
P02768
gi|28,592
17
866
/71,316
/6.05
down 16,67
      

Serum albumin

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Nov 3, 2016 | Posted by in NEPHROLOGY | Comments Off on Hormone-Dependent Changes in Female Urinary Proteome

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